The sd-LDL
SEIKEN assay quantitatively determines small, dense low-dense lipoproteins
(sd-LDL), a subfraction of LDL associated with an increased risk for cardiovascular
disease (CVD). Past studies have shown that people with a predominance
of sd-LDL (so called Pattern B patients) have a 3-fold increased risk
of myocardial infarction (MI), while the relative risk is 4.5 for CAD
and 7 for MI when sd-LDL levels are greater than 100mg/dL. Furthermore,
the sd-LDL assay offers a great potential for standardization compared
to current methods, which are disparate and require considerable technical
expertise to perform. This assay filters out large, buoyant LDLs and other
lipoproteins by forming aggregates with the polyanion and divalent cation-based
reagent in a simple off-line precipitation step. The second reagent then
measures small, dense LDL cholesterol on automated chemistry analyzers.
The assay offers linearity up to 180 mg/dL, good correlation to ultracentrifugation
methods, and coefficients of variation of less than 5%, while taking only
30 minutes to perform.
NOTE: sd-LDL "SEIKEN" assay is currently not available for sale
in the United States.
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